两种BA—ELISA法和SPT法检测尘螨过敏原比较分析研究

Comparison Analysis of Dust Mite Specific with Two BA-ELISA and SPT

目的采用北京地区和广州地区过敏原包被研制出的过敏原体外检测试剂盒检测变应性疾病患者血清中尘螨过敏原特异性IgE抗体，并将其结果做比较分析。方法分别采用北京地区过敏原（BA—ELISA法一组）和广州地区过敏原（BA—ELISA法二组）作为包被抗原的过敏原体外检测试剂盒对800例过敏性鼻炎患者进行体外检测其过敏原，并将其中尘螨检测结果分别与尘螨皮肤点刺试验（SPT法）结果进行比较分析。结果两种采用北京地区争广州地区过敏原包被研制出的过敏原体外检测试剂盒检测尘螨阳性率分别为30．2％和32．0％，与SPT法试验阳性率结果比较，彼此差异无统计学意义。结论采用北京和广州不同地区过敏原包被研制出的过敏原体外检测试剂盒没有明显的地域性，均可用于不同地区过敏性疾病的检测。

Objective To study comparative analysis of dust mite specific IgE in patients with allergic diseases with different methods. Allergens from Beijing district and Guangzhou district coated antigens in these enzyme-linked immunosorbent assays. Methods Allergen-specific IgE antibody in 800 patients with allergie rhinitis were detected using two enzymelinked immunosorbent assay combined with biotin-avidin signal amplification system （BA-ELISA method）. Allergens from Beijing district and Guangzhou district coated antigens in these enzyme-linked immunosorbent assays. The results of dust mites were compared with skin prick test （SPT method） results. Results The positive rates of two BA-ELISA kits were 30. 2% and 32.0% respectively,compared with SPT method,there was no significant difference. Conclusion Allergens from different regions of China as the coating antigen in detection kit has no significant regional. It can be used for the detection of allergic diseases in different regions.