卵泡直径对辽宁绒山羊卵母细胞体外培养的影响

Effects of Follicle Size on Developmental Competence of Liaoning Cashmere Goats Oocytes Following Maturation,Fertilization and Culture in vitro

本试验以屠宰场绒山羊卵巢为材料,采用抽吸法收集不同直径卵泡卵母细胞,研究卵泡直径对卵母细胞回收效果和体外成熟、体外受精的影响。结果表明,卵泡直径直接影响卵母细胞的回收效果及随后的体外成熟、体外受精;卵母细胞的回收率和可用卵的比率及成熟率与卵泡直径呈正相关;直径2.1～5 mm卵泡的卵母细胞由于处于生长旺盛期,受精率和卵裂率分别为59.15%和42.50%,显著高于直径>5 mm卵泡和≤2 mm卵泡,适合体外胚胎的生产,而直径≤2 mm卵泡的卵母细胞因未发育充分体外发育潜能最差;直径>5 mm卵泡的卵母细胞可能因体外培养成熟过度而老化导致受精率和卵裂率降低,缩短其体外成熟时间可能会提高其体外发育潜能。

This experiment was carried out to study effects of follicle size on collecting effect and developmental competence of goats oocytes derived from abattoir ovaries following maturation,fertilization and culture in vitro.In this experiment,the aspiration method was applied to collect the follicular oocytes.The results showed that follicle size directly affected collecting effect of oocytes and development of oocytes after IVMFC.The rate of collecting oocytes,the avaible oocyte and maturation was interrelated with the diameter of follicular positively.Both fertilization and cleavage rates of oocytes from 2.1 to 5 mm follicles were significantly higher than from 〉5 mm follicles and ≤2 mm follicles,which were suitable for embryo in vitro production.The oocytes from ≤2 mm follicles had the lowest developmental competence in vitro.Both fertilization and cleavage rates of oocytes from 〉5 mm follicles decreased ascribed to oocytes degeneration.We could improve the developmental competence of oocytes from 〉5 mm follicles by decreasing the cultural time in vitro.