摘要
本试验通过对比冷冻保护剂和冷冻程序,优化犬精液冷冻保存方法,比较了不同甘油、乙二醇浓度、不同平衡时间对犬精子冷冻复苏率及精子顶体完整率的影响。结果发现,甘油作为冷冻保护剂时,其对精液冷冻保存后的效果明显优于乙二醇,当甘油浓度为4%时,平均冷冻复苏率最高;冷冻平衡时间为1 h最佳。因此,犬精液在冷冻保存时,甘油浓度为4%,平衡60 min,可获得平均39.21%±0.013%的冷冻复苏率,且比较稳定。
In this study,the dog semen was frozen with different cryopreservation,including glycerol and glycol,for better cryopreservation result.It was conducted to compare different concentration of glycerol or ethylene glycol's effects on frozen sperm's recovery rates and compare different balance time.The glycerol as cryoprotectant is better than ethylene glycol and the concentration of glycerol is 4%,the average recovery rate of frozen semen is highly,60 min of the balance time was more helpful to the survival of frozen sperm.The study found that cryopreservation at 4% of glycerol,balance 60 min at 4 ℃ achieved better sperm recovery for canine semen cryopreservation,with an average of 39.21%±0.013%recovery rate of frozen semen,and the results were rather stable.
出处
《中国畜牧兽医》
CAS
北大核心
2010年第2期122-124,共3页
China Animal Husbandry & Veterinary Medicine