顶空气相色谱法测定赤芍提取物中因大孔吸附树脂带入的有机残留物

Determination of organic residues with macroporous adsorptive resin in Extract of Radix Paeoniae Rubra P. E by head - space gas chromatography

目的：建立赤芍提取物中苯、甲苯和二乙烯苯有机残留物的检测方法。方法：采用顶空气相色谱法，用AgilentHP-5毛细管柱（30m×0．320mm×0．25μm）为色谱柱，氢火焰离子化检测器（HD），程序升温，进行顶空气相色谱测定。结果：苯、甲苯和二乙烯苯在所考察的浓度范围内具有良好的线性，相关系数R分别为0．9987、0．9997和0．9971；平均回收率分别为98．0％、102．8％和99．6％（RSD=2．26％、2．30％和3．65％，n=9）。结论：该方法简便易行，测定结果准确可靠，可用于赤芍提取物中大孔吸附树脂残留物的检测。

Objective：To establish the method for determination of several organic residues of benzene, methylbenzene and divinyl- benzene in Extract of Radix Paeoniae Rubra P. E. Methods ： Using an HP - 5 capillary column, with nitrogen as carrier gas and FID as detec- tor. The injector temperature was 200℃ and the detector temperature was 250℃, temperature program and split injection mode were used. Results ： Benzene, methylbenzene and divinylbenzene showed a good linearity within the designed range of each solvent, and their related co- efficient were 0.9987 、0.9997 and 0.997 1, respectively. The average recoveries were 98.0%, 102.8 % and 99.6% （ RSD = 2.26% ,2.30% and 3.65 % , n = 9 ）, respectively. Conclusion ： The method is accurate and quick with the high sensitivity for the determination of organic residues with macroporous adsorptive resin of Extract of Radix Paeoniae Rubra P. E.