期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

P-LISA技术

P-LISA
原文传递
导出
摘要 原位邻近式连接分析(proximity ligation in situ assay,P-LISA)是一种用来研究蛋白质-蛋白质相互作用的新方法。该方法能够对原位、瞬时、微弱的蛋白质-蛋白质相互作用进行定量分析和亚细胞定位,在药物研发和临床诊断中将有着重要的应用价值。 Proximity ligation in situ assay （P-LISA） is a new technique to examine protein-protein interactions, which can provide quantitative information and subcellular localization of protein-protein interactions and report weak and transient protein-protein interactions in situ. In future, P-LISA will be of great importance in the drug development and clinical diagnostics.
作者 李恩 齐锦生
机构地区 河北省人民医院西医眼科 河北医科大学生化教研室
出处 《生命的化学》 CAS CSCD 北大核心 2010年第1期132-134,共3页 Chemistry of Life
关键词 P-LISA 邻近式连接 蛋白质一蛋白质相互作用 proximity ligation in situ assay （P-LISA） proximity ligation protein-protein interaction
分类号 Q503 [生物学—生物化学]
  • 相关文献

参考文献13

  • 1Fredriksson S. Visualizing signal transduction pathways by quantifying protein-protein interactions in native cells and tissue. Nat Methods, 2009, 6:1-2.
  • 2Soderberg O et al. Direct observation of individual endogenous protein complexes in situ by proximity ligation. Nat Methods, 2006, 3:995-1000.
  • 3Fredriksson S et al. Protein detection using proximity-dependent DNA ligation assays. Nat Biotechnol, 2002, 20:473-477.
  • 4Gullberg M et al. Cytokine detection by antibody-based proximity ligation. Proc Natl Acad Sci USA, 2004, 101:8420-8424.
  • 5Schallmeiner E et al. Sensitive protein detection via triplebinder proximity ligation assays. Nat Methods, 2007, 4: 135- 137.
  • 6Gustafsdottir SM et al. In vitro analysis of DNA-protein interactions by proximity ligation. Proc Natl Acad Sci USA, 2007, 104:3067-3072.
  • 7Gustafsdottir SM et al. Proximity ligation assays for sensitive and specific protein analyses. Anal Biochem, 2005, 345:2-9.
  • 8Gullberg M et al. A sense of closeness: protein detection by proximity ligation. Cur Opin Biotechnol, 2003, 14:82-86.
  • 9Soderberg O et al. Characterizing proteins and their interactions in cells and tissues using the in situ proximity ligation assay. Methods, 2008, 45:227-232.
  • 10刘先早.蛋白质相互作用研究的技术方法和应用[J].西南国防医药,2009,19(2):251-253. 被引量：7

二级参考文献24

  • 1ITO T,TASHIRO K,MUTA S,et al. Toward a protein - protein inter - action map of the budding yeast:a comprehensive system to examine two - hybrid interactions in all possible combinations between the yeast proteins[J]. Proc Natl Acad Sci USA,2000,97(3):1143 - 1147.
  • 2TERRADOT L,DURNELL N,LI M,et al. Biochemical characterization of protein complexes from the Helicobacter pylori protein interaction map:strategies for complex formation and evidence for novel interactions within type Ⅳ secretion systems[J]. Mol Cell Proteomics, 2004,3(8) :809 - 819.
  • 3RAIN J C,SELIG L,DE REUSE H,et al. The protein - protein interaction map of Helicobaeter pylori [ J ]. Nature,2001,409 (6817) :211 - 215.
  • 4ITO T, CHIBA T, OZAWA R, et al. A comprehensive two - hybrid analysis to explore the yeast protein interaetome[ J]. Proc Natl Acad Sci USA,2001,98 (8) :4569 - 4574.
  • 5GIOT L, BADER J S, BROUWER C, et al. A protein interaction map of Drosophila rnelanogaster[ J ]. Science, 2003,302 ( 5651 ) : 1727 - 1736.
  • 6LI S,ARMSTRONG C M,BERTIN N,et al. A map of the interactome network of the metazoan C elegans[J]. Science,2004,303(5657):540 - 543.
  • 7RU AL J F,VENKATESAN K, HAO T,et al. Towards a proteome - scale map of the human protein - protein interaction network [J]. Nature, 2005,437(7062) :1173 - 1178.
  • 8STELZL U, WORM U, LALOWSKI M, et al. A human protein - protein interaction network : a resource for annotating the proteome [J]. Cell,2005,122(6) :957 - 968.
  • 9MILLER J, STAGLJAR I. Using the yeast two - hybrid system to identify interacting proteins[ J]. Methods Mol Biol,2004,261:247 - 262.
  • 10CHO S,PARK S G,LEE D H,et al. Protein - protein interaction networks :from interactions to networks [ J ]. J Biochem Mol Biol,2004,37 ( 1 ) :45 - 52.

共引文献6

生命的化学
2010年 第1期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部