霉酚酸在肝移植受者的药代动力学与药效学相关性研究

Relationship between pharmacokinetics and pharmadynamics of mycophenolic acid in liver transplant recipients

目的研究肝移植受者早期口服霉酚酸(免疫抑制剂)药代动力学与药效学的相关性。方法20例肝移植受者口服霉酚酸酯前、后,用酶增强免疫法测定患者血浆霉酚酸浓度。服药前(0h)及服药后1,2h,用患者血清处理人急性淋巴细胞白血病T淋巴(CEM)细胞,检测CEM细胞的增殖情况。结果口服霉酚酸酯前,CEM细胞增殖率显著高于服药后1,2h(P<0.05);服药后1,2h霉酚酸浓度与相应的CEM细胞增殖率呈负相关(P<0.05)。结论肝移植受者口服霉酚酸酯后,血清对CEM细胞的增殖产生显著抑制作用,其作用和霉酚酸浓度呈显著负相关。

Objective To investigate the relationship between phannacokinetics and pharmadynamics of mycophenolic acid （MPA） in liver transplant recipients. Methods Plasma MPA concentration were determined EMIT method before MMF dose and after dosing in 20 liver transplant patients received mycophenolate mofetil （MMF）. The inhibitory capacity of serum MPA on proliferation of CEM cells was also monitored at 0, 1 h and 2 h. Results The rate of proliferation of CEM cells before MMF administration was higher significantly than those at 1 h and 2 h after dosing （P 〈0.05 ）. The concentrations of MPA at 1 h and 2 h were conversely related with the rate of proliferation of CEM cells （ P 〈0.05）. Conclusion After MMF dose, the inhibitory capacity of serum MPA on proliferation of CEM cells was enhanced significantly. This effect was related greatly to MPA concentrations.