摘要
目的:建立柚皮苷固体脂质纳米粒(solid lipid nanoparticles,SLN)的包封率和体外释放度的测定方法。方法:采用超滤法和高效液相色谱法测定柚皮苷SLN包封率。通过透析袋法测定柚皮苷SLN的体外释放度。结果:柚皮苷在2.5～160.0μg·ml^(-1)范围内,线性关系良好(r=0.999 9)。平均回收率为99.9%,RSD为1.1%。透析袋对柚皮苷没有吸附作用。柚皮苷SLN包封率为61.3%,体外释药曲线符合一级动力学方程。结论:所建立的方法简单可靠。
Objective： To develop a method for determination of encapsulation efficiency and release rate in vitro of naringin solid lipid nanoparticles （SLN）. Method ： Ultrafiltration and HPLC method were used to determine naringin SLN encapsulation efficiency. Dialysis bag was applied to detect release rate in vitro. Result： The linear range of naringin was 2. 5-160 μg· ml^-1 （r = 0. 999 9 ）. The average recovery was 99. 9% （RSD = 1.7% ）. Dialysis bag did not absorb naringin. The encapsulation efficiency of naringin SLN was 61.3 % and release rate in vitro conformed to first-order kinetics equation. Conclusion： The method is simple and reliable.
出处
《中国药师》
CAS
2010年第3期318-320,共3页
China Pharmacist
基金
湖北省自然科学基金(2008CD243)
武汉药学会默沙东青年药师科研基金
关键词
柚皮苷
固体脂质纳米粒
超滤法
包封率
体外释放度
Naringin
Solid lipid nanoparticles
Uhrafihration
Encapsulation efficiency
release rate in vitro
