摘要
目的:建立HPLC测定复方积雪草苷凝胶剂中积雪草苷含量的方法。方法:采用Hypersil ODS柱(250 mm×4.6mm,5μm),流动相:甲醇-水(58:42),流速:1.0 ml·min^(-1),进样量:20μl,检测波长:205 nm,柱温:35℃。结果:积雪草苷浓度在0.05～0.90 mg·ml^(-1)范围内峰面积与浓度呈良好线性关系(r=0.999 7),平均回收率为99.05%,RSD为0.62%。结论:本方法操作简便,精密度高,重现性好,结果准确,可作为复方积雪草苷凝胶剂中积雪草苷的含量测定方法。
Objective： To establish a method for determination of asiatieoside in Compound Asiatieoside Gel by HPLC. Method： Chromatography was performed on a Hypersil ODS column （250 mm ×4. 6 mm,5 μm） at 35 ℃ with a mobile phase consisted of methanal-water （58：42） ,flow rate was 1.0 ml·min^-1. The injection volume was 20 μl and the UV detection wavelength was 205 nm. Result： A good linear relationship between peak area and concentration was obtained at the concentration range of 0. 05 - 0. 90 mg· ml ^-1 （ r = 0. 999 7 ）. The average recovery was 99. 05 % with a relative standard deviation of 0. 62 %. Conclusion： The method is simple, precise, replieable and accurate, which can be used to determine the content of asiatieoside in Compound Asiatieoside Gel.
出处
《中国药师》
CAS
2010年第3期336-337,共2页
China Pharmacist
基金
广州市中医药中西医结合科研课题(2005B026)
