期刊文献+

枯草芽孢杆菌TKPG011聚谷氨酸发酵培养基的优化 被引量:5

Medium Optimization for Poly-γ-glutamic Acid Production by Bacillus subtilis TKPG011
下载PDF
导出
摘要 本文通过单因素试验和正交实验设计研究了碳源、氮源、前体物L-谷氨酸钠和初始pH对Bacillus subtilis TKPG011生物合成γ-PGA的产量的影响,结果表明优化后的发酵培养基为:葡萄糖50g/L,酵母膏10g/L,L-谷氨酸钠30g/L,初始pH值6.5,K2HPO41.5g/L,MgSO4·7H2O0.5g/L。采用优化培养基能使γ-PGA发酵产量达9.20g/L。 The optimal fermentation medium for producing 7-PGA by a mutant strain Bacillus subtilis TKPGO11 was studied by single-factor and oahogonal design theory. With shaking-flask experiments, the optimal fermentation medium consisted of 50 g/L glucose, 10 g/L yeast extract, 30 g/L L-Glutamate, 1.5 g/L K2HPO4 and 0.5 g/L MgSOa.7H20 at pH 6.5. With this medium, the yield of γ-PGA in the shaking-flask could be up to 9.20 g/L.
出处 《现代食品科技》 EI CAS 2010年第3期253-255,共3页 Modern Food Science and Technology
基金 天津市科技支撑重点项目(09ZCKFSH00800) 天津滨海新区双百科技特派员科技专项(SB20080039)
关键词 Γ-PGA 枯草芽胞杆菌 培养基 优化 γ-PGA Bacillus subtilis, medium optimization
  • 相关文献

参考文献8

  • 1Yoshihiro O, Hiroshi H, Mitsutoshi H, et al.Purification and properties of γ-glutamyltranspeptide from Bacillus subtilis (natto) [J]. Agric. Biol. Chem., 1991, 55(12): 2971-2977.
  • 2Oppemann-Sanio F B, Alexander S. Occurrence, functions and biosynthesis of polyamides in microorganisms and biotechnological production[J].Natrurwi-ssenschaflen,2002,89 11-22.
  • 3Yoshihiro O, Fumio Y, Katsumi Y, et al.Efficient production of γ-polyglutamic acid by Bacillus subtilis(natto)in Jar Fermemers[J]. Biosci. Biotechnol. Biochem., 1997,61(6):1684- 1687.
  • 4Jin H D, Chang H N, Lac S Y, et al. Efficient recovery of γ-poly(glutamic acid) from hight viscous culture broth[J]. Biotechnology and Bioengineering, 2001,76(3):219-224.
  • 5徐艳萍,王树英,李华钟,陈坚.聚γ-谷氨酸高产突变株的选育及摇瓶发酵条件[J].无锡轻工大学学报(食品与生物技术),2004,23(5):6-10. 被引量:18
  • 6Kubota H, Matsunobu T, Uotani K, et al. Production of poly(γ-polyglutamic acid) by Bacillus subtilis F-2-01[J]. Biosci. Biotechnol. Biochem.,1993,57:1212-1213.
  • 7Kunioka M, Goto A, Biosynthesis of poly (γ-polyglutamic acid) from L-glutamic acid, citric acid and ammonium sulfate in Bacillus subtilis IFO 3335[J].Appl. Microbiol. Biotechnol., 1994,40:867-872.
  • 8张宁,旷弛.利用豆粕发酵生产聚谷氨酸的研究[J].现代食品科技,2008,24(2):113-114. 被引量:5

二级参考文献9

  • 1[4]Makoto Ashiuchi,Hisaaki Nakamura,Takashi Yamamoto,Journal of Molecular Catalysis B:Enzymatic,2003,23:249-255
  • 2[1]Shih,I L,Van Y T.Bioresource Technology,2001,79:207-2251
  • 3[2]Ho-Nam C,Sang-Yup L,Jin Hwan D,et al.US:2001/001634 1A1,2001
  • 4Kim K S, Kim T K, Graham N B. Controlled release behavior of prodrugs based on the biodegradable poly(γ-glutamic acid) microspheres[J]. Polymer Journal, 1999,31 : 813- 816.
  • 5Choi H J, Kunioka M, Preparation conditions and swelling equilibria of hydrogel prepared by (γ-irradiation from microbial poly(γ-glutamic acid) [J]. Radial Phys Chem, 1995, 46 (2) : 175- 179.
  • 6YasuyoshiA YujiF ShuichiK.Health food with poly(γ-glutamic acid) as the chief ingredient [P].日本专利:5095767A2.1993-4-20.
  • 7Yahata K, Sadanobu J, Endo T. Preparation of poly-benzyl-γ-glutamic acid fiber[J]. Polym Prepr Jpn, 1992, 42: 1077.
  • 8Goto A, Kunioka M. Biosythesis of poly(γ-glutamic acid) from L-glutamic acid, citric acid, and ammonium sulfate in Bacillus subtilis IFO3335[J]. Applied Microbiology Biotechnology, 1994, 40:867-872.
  • 9Elizabeth C, King A, Blacker J, et al. Enzymatic breakdown of poly-γ-D-glutamic Acid in Bacillus licheniformis : identification of a polyglutamyl γ-hydrolase enzyme[J]. Biomacromolecules, 2000,1: 75-83.

共引文献21

同被引文献57

引证文献5

二级引证文献13

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部