枯草芽孢杆菌TKPG011聚谷氨酸发酵培养基的优化

Medium Optimization for Poly-γ-glutamic Acid Production by Bacillus subtilis TKPG011

本文通过单因素试验和正交实验设计研究了碳源、氮源、前体物L-谷氨酸钠和初始pH对Bacillus subtilis TKPG011生物合成γ-PGA的产量的影响,结果表明优化后的发酵培养基为:葡萄糖50g/L,酵母膏10g/L,L-谷氨酸钠30g/L,初始pH值6.5,K2HPO41.5g/L,MgSO4·7H2O0.5g/L。采用优化培养基能使γ-PGA发酵产量达9.20g/L。

The optimal fermentation medium for producing 7-PGA by a mutant strain Bacillus subtilis TKPGO11 was studied by single-factor and oahogonal design theory. With shaking-flask experiments, the optimal fermentation medium consisted of 50 g/L glucose, 10 g/L yeast extract, 30 g/L L-Glutamate, 1.5 g/L K2HPO4 and 0.5 g/L MgSOa.7H20 at pH 6.5. With this medium, the yield of γ-PGA in the shaking-flask could be up to 9.20 g/L.