高纯度玉米黄素的制备

Preparation of High-purity Zeaxanthin

本文研究了氢氧化钾(KOH)与叶黄素的摩尔比例、KOH水溶液的摩尔浓度、反应温度、料液比四个因素对叶黄素转化为玉米黄素反应的影响。实验结果表明,当KOH和叶黄素的摩尔比为56:1,KOH水溶液摩尔浓度为12M,料液比为1:25,温度为90℃,反应时间为1h,叶黄素转化效率最高,经过重结晶,所得玉米黄素的含量可达90%以上,总收率在60%以上。

Effects of KOH-lutein ratio, concentration of KON solution, temperature and liquid-solid ratio on the synthesis of zeaxanthin using lutein were studied. The results show that the best reaction condition were as follows： lutein-alkali hydroxide ratio of 1/56, the concentration of aqueous alkali hydroxide solution of 12M, temperature of 90 ℃ and the material-solvent ratio of 1/25. HPLC analysis showed that the purity of zeaxanthin after recrystallization was above 90%, and its yield reached 60%.