NADPH氧化酶DUOX1在肝细胞癌中的表达及临床意义

Expression and significance of NADPH oxidase DUOX1 in hepatocellular carcinoma after tumor resection

目的探讨NADPH氧化酶DUOX1在肝细胞癌(HCC)中的表达及其与临床病理指标及预后的关系。方法选取7株人HCC细胞系及1株人正常肝细胞系、30例正常肝组织及103例肝癌标本作为研究对象,采⒚RT-PCR的方法研究DUOX1 mRNA的表达情况,结合临床病理指标及预后进行分析。结果DUOX1 mRNA在MHCC-97H、MHCC-97L及BEL7402细胞系中表达,在HepG2、Hep3B、SMMC-7721、Chang liver和LO2中无表达。30例正常肝组织中均无表达。在肝癌和癌旁肝组织中表达阳性率分别为53.4%(55/103)和14.5%(15/103),差异有高度统计学意义(P<0.01)。相关性统计分析提示肝癌组织中DUOX1 mRNA表达状态与性别、年龄、有无卫星结节及术前AFP水平相关。单因素分析提示影响肝癌术后总体生存率的因素为性别、年龄、肿瘤直径、有无卫星结节、有无门静脉癌栓、TNM分级以及DUOX1 mRNA表达状态。多因素分析提示影响肝癌术后总体生存率的因素为肿瘤直径、有无门静脉癌栓及DUOX1 mRNA表达状态。结论肝癌组织DUOX1 mRNA表达状态是肝癌术后总体生存的独立预后因素,DUOX1基因可能与肝癌的发生发展有关。

Objective To study the prognostic value of dual oxidase 1 （DUOX1） gene in hepatocellular carcinoma（HCC）. Methods Reverse transcription polymerase chain reaction RT-PCR） analysis were used to detect the expression of DUOXI mRNA in 7 HCC cell lines, one normal liver cell line, specimen of normal liver tissues in 30 cases and paired specimen of neoplastic with matched adjacent non-neoplastic liver tissues in 103 cases. The correlation of the DUOX 1 mRNA expression in neoplastic tissues were analyzed with clinicopathological and prognostic factors. Results DUOX1 mRNA was expressed in MHCC-97H, MHCC-97L and BEL7402 cell lines, but not in HepG2, Hep3B, SMMC-7721, Chang liver and LO2 cell line. The expression of DUOX1 mRNA was not found in all 30 normal liver tissues. The positive rates of DUOX1 expression in neoplastic tissues was higher than in adjacent non-neoplastic tissues（53.4% vs 14.5%, P 〈0.01）, respectively. Univariate analysis indicated that factors affecting overall survival were gender, age, tumor size, satellite nodule, tumor thrombus in the portal vein, TNM stage and DUOX1 expression status in neoplastic tissues. Multivariate analysis indicated that independent factors affecting overall survival were tumor size, tumor thrombus in the portal vein and DUOX1 expression status. Conclusions DUOX1 expression in HCC neoplastic tissues is an independent factor affecting the prognosis of patients with HCC after tumor resection. DUOX 1 may play a role in liver carcinogenesis.