城市污水中气味类香菌的分离与鉴定

Isolation and Identification of Myroides odoratus in Municipal Sewage

目的分离并鉴定城市污水中的病原细菌——气味类香菌。方法采用选择性培养基细菌分离方法,从城市污水中分离出菌株JD23,利用16SrDNA分子生物学技术和生物信息学技术,通过16SrDNA序列分析,构建系统发育树,结合常规生理生化实验和形态学观察鉴定菌株种属,并确定其分类学地位。结果该菌株与Myroides odoratus M58777.2相似度达98%,结合常规生理生化实验和形态学观察,进一步确定JD23为气味类香菌。结论选择性培养基结合16SrDNA序列分析方法可分离并鉴定城市污水中的气味类香菌。

Objective To isolate and identify the pathogenic bacterial strain Myroides odoratus from municipal sewage in Zhenjiang city. Methods With selective medium,a pathogenic bacterial strain JD23 was isolated from the city sewage. Combining the modem molecular biology technology methods and bioinformatics methods,its taxonomic status was confirmed by 16S rDNA gene sequence homologous analysis and phylogenetic tree. The traditional experiments of physiology, biochemistry and morphology were also studied. Results Strain JD23 exhibited the highest levels （98%） of similarity to the M57oides odoratus M58777.2. Combining all the results of several methods and morphological observation,strain JD23 was finally identified as the bacterial classification Myroides odoratus. Conclusion There was a pathogenic microrganism,Myroides odoratus,in municipal sewage of Zhenjiang city.