饮用水中双酚A的固相萃取-高效液相色谱-电喷雾电离串联质谱测定法

Method for Determination of Bisphenol A in Drinking Water by Liquid Chromatography-Tandem Mass Spectrometry

目的建立固相萃取-液相色谱-电喷雾电离串联质谱测定饮用水中双酚A的方法。方法水样用SEP-PAKC18进行富集,甲醇淋洗,旋转蒸发浓缩后,采用高效液相色谱-负离子电喷雾电离串联质谱法测定。结果双酚A在5～100ng/ml线性范围内,得到回归方程y=6796.61x-8655.64,相关系数为0.9992,检测限(S/N=3)为0.0075ng/ml,定量下限(S/N=10)为0.025ng/ml。该方法的平均回收率为83.46%～94.00%,RSD为3.06%～4.60%。结论该方法简便、准确、快速,适用于水中双酚A的检测。

Objective To develop a rapid and sensitive method for the determination of bisphenol A in drinking water by liquid chromatography-electrospray tandem mass spectrometry. Methods Bisphenol A was extracted from the drinking water sample by a SEP-PAK C18 column, eluted with methanol and concentrated with a rotary evaporator. The extract was analyzed by liquid chromatography-tandem mass spectrometry with electrospray ionization. Results The calibration curve of bisphenol A was linear in the range of 5-100 ng/ml, the linear equation was y=6 796.61x-8 655.64 and the correlation coefficients of linear calibration curve was 0.999 2. The limit of detection （S/N=3） and the limit of quantification （S/N=10） of bisphenol A were 0.007 5 ng/ml and 0.025 ng/ml, respectively. The rates of recovery of bisphenol A were from 83.46% to 94.00% and the relative standard deviation was 3.06%-4.60%. Conclusion The method is simple,rapid, accurate and is applicable to the qualitative and quantitative determination of bisphenol A in drinking water.