C3A细胞系构建生物人工肝治疗猪急性肝衰竭研究

Effect of C3A Cells Derived Bioartificial Liver Support System on Fulminant Hepatic Failure Pigs

目的探讨应用C3A细胞系所构建的生物人工肝对猪急性肝衰竭动物模型的治疗效果。方法将12只中华实验猪颈外静脉注射D-氨基半乳糖以建立急性肝衰竭模型,随机分成对照组(n=6)与生物人工肝治疗组(n=6)。应用C3A细胞系构建体外生物人工肝支持系统,在给予D-氨基半乳糖24h后对治疗组进行连续36h治疗,监测治疗前后不同时间点两组动物的一般状况、生存时间和生化指标。结果对照组动物平均存活时间为(48.9±11.2)h,治疗组存活时间为(105.7±12.6)h,生物人工肝治疗组在给药后48h、72h、96h、120h等各个时间点动物生存率均大于对照组。生物人工肝治疗结束时,血清胆红素、转氨酶下降,白蛋白、凝血酶原活动度上升,与对照组比较,差异有显著性(P<0.05～P<0.01);血氨下降差异不明显(P>0.05)。结论D-氨基半乳糖猪急性肝衰竭模型能够较好的模拟临床急性肝衰竭过程,C3A细胞系生物人工肝具有一定的肝脏合成和解毒功能。

Objective To investigate the effects of C3A cells derived bioartificial liver support system on fulminant hepatic failure pigs. Method Construct the extraeorporeal liver support system with C3A cells, then the treatment group was given 36 hours of continuous treatment after D - galactosamine were injected 24 hours later. Observe general condition, survival time and related biochemical parameters of two groups at different time points. Results There were significant difference of general condition, survival time and related biochemical parameters between two groups. Conclusion D -galaetosamine -induced fulminant hapetic failure of pigs were able to simulate the clinical process of acute hepatic failure, C3A cells derived bioartificial liver support system have some liver synthesis and detoxifieation function.