利用AFLP分子标记鉴定新疆梨的分类地位

Classification Position of Xinjiang Pear Identified by AFLP

[目的]为新疆梨的分类地位提供DNA水平上的依据。[方法]利用AFLP分子标记技术,通过聚类分析和遗传相似系数分析,对新疆梨进行分类研究。[结果]当阈值为15时,新疆梨品种兰州长把和花长把首先聚在一起,然后再与西洋梨品种巴梨、红巴梨、红茄、茄梨、保利阿斯卡、朱丽比恩和新疆梨品种其力阿木提聚在一起。7个新疆梨品种中,群体内品种之间欧式距离为2.646～10.050,与砂梨系统、西洋梨系统、白梨系统、秋子梨系统各品种间最小欧式距离分别为7.746、7.746、7.810、8.165。[结论]新疆梨与西洋梨系统有较近亲缘关系。

[Objective] The aim was to provide DNA level basis for Xinjiang Pear classification position.[Method] Through cluster analysis and genetic similarity coefficient analysis,the classification study on Xinjiang Pear was carried out by using AFLP molecular marker technique.[Result] When the threshold value is 15,Xinjiang Pear cultivar Lanzhouchangba hold together with Huachangba first,then with Pyrus communis L.cultivars Bali,Hongbali,Hongqie,Qieli,Baoli＇asika,Zhulibi＇en and Xinjiang Pear cultivar Qili＇amuti.Among seven Xijiang Pear cultivars,the euclidean distance among species within the group ranged from 2.646 to 10.050.And the smallest euclidean distance between Xinjiang Pear and P.Bretschneideri Rehd,P.communis L.,P.ussuriensis Maxim,and P.pyrifolia Nakai were 7.746、7.746、7.810、8.165 respectively.[Conclusion] Xinjiang Pear has the closest relationship with P.communis L..