3种不同耐寒番茄品系的生理特性研究

Study on Physiological Characters of Three Different Cold-resistant Tomato Strains

[目的]研究自然条件下选育的3种不同耐寒番茄品系的耐寒指标及其生理特性。[方法]以苗龄21～28 d的番茄幼苗为材料,通过实验室冷处理模拟外界环境条件,鉴定植株的存活及生长状况;研究植株离体叶片冷处理恢复后的形态变化并测定相对电导率,鉴定不同品系番茄植株的耐寒程度,分析电导率与存活率的相关性;测定冷胁迫条件下番茄植株体内可溶性糖和脯氨酸含量变化。[结果]不同品系的番茄冷处理后,叶片形态变化、电导率大小、恢复后的生长状况存在较大差异;不同品系番茄植株体内可溶性糖含量变化差异显著,脯氨酸含量变化差异不显著。[结论]冷处理条件下不同品系番茄叶片的电导率与存活率存在一定相关性,植株耐寒性与可溶性糖含量的积累有关。

[Objective] The aim was to study cold-resistant indexes and physiological characters of three different cold-resistant tomato strains selected under natural conditions.[Method] With 21-28 days tomato seedlings as tested materials,through simulating external environment conditions by the cold treatment in lab,the survival ratio and growth status of seedlings were identified;by studying the morphological change of vitro leaves after cold treatment and determining the relative conductivity,the cold-resistant degree of different tomato strains was identified and the correlation between conductivity and survival rate was analyzed;the variation of soluble sugar and proline content in plant of tomato was determined under the condition of cold stress.[Result] The results showed that leaf morphological changes,size of conductivity and growth status of different tomato strains after cold treatment have significant differences;the variation of soluble sugar contents in plant has significant differences,while the variation of proline content has no significant differences.[Conclusion] There is a certain correlation between conductivity and survival rate of different tomato leaves under cold treatment conditions,and plant cold tolerance is related to accumulation of soluble sugar content.