白花除虫菊四倍体与二倍体株系抗氧化酶活性的研究

Research on the Antioxidase Activities of Tetraploid Lines and Diploid Control Line of Chrysanthemum cinerariifolium

[目的]测定白花除虫菊四倍体株系与二倍体对照株系试管苗和田间叶片中SOD、POD及APX酶的活性,为选育抗性强的白花除虫菊优良四倍体品种提供理论依据。[方法]分别测定样品的SOD、POD及APX活性,每种材料取3份测定,求平均值,进行分析。[结果]四倍体株系与二倍体对照株系比较,其保护酶系统中各种抗氧化酶的活性普遍都能得到提高,并且各株系的田间样品与试管苗样品间具有一定的相关性。[结论]试管苗期间各株系的抗氧化酶活性可以作为筛选抗病抗逆性强的白花除虫菊优良株系的参考指标。

[Objective] The SOD,POD and APX enzyme activities of tetraploid lines and diploid control line of Chrysanthemum cinerariifolium which were in test-tube and in flied were determined in order to provide a reference for the selective breeding of high resistance good tetraploid varieties of C.cinerariifolium.[Method] The SOD,POD and APX activities of each sample were determined.Every material should have 3 replicates which been determined and calculated mean value to analyze.[Result] The various kinds of antioxidase activities in protective enzyme system of tetraploid lines were increased generally in comparison with that of diploid control line.And there is a certain relativity between the samples in field and in test-tube of these lines.[Conclusion] The antioxidase activities of each line in test-tube could be considered as reference index to screen high resistance good tetraploid varieties of C.cinerariifolium.