紫外分光光度法测定发酵液中卑霉素的含量

Determination on Avilamycin Content in Fermentation Liquor by Using UV spectrophotometry

[目的]介绍利用紫外分光光度法测定发酵液中卑霉素含量的方法。[方法]以乙酸乙酯为提取剂,扫描卑霉素的乙酸乙酯溶液在200～400 nm的吸收峰,准确配制出系列浓度的卑霉素乙酸乙酯溶液,绘制标准曲线,在最佳波长下进行测定,然后对发酵样品进行3次离心处理得到样品溶液,再对样品液进行重现性、稳定性的研究和回收率的计算,用抑菌圈法验证样品的抑菌效果。[结果]选择275nm波长作定量峰,卑霉素的乙酸乙酯溶液浓度在0.5～12.0 mg/L范围内、pH值为4.6的条件下,其吸收度与浓度的线性关系良好,线性回归方程y=15.968 0x+0.030 7,相关系数为0.995 5。样品溶液在室温条件下放置24 h保持仍稳定,平均回收率为102.13%。[结论]试验证明,用紫外分光光度计法检测发酵液中的卑霉素含量是可行的。

[Objective] The aim was to introduce the method of determining the avilamycin content in fermentation liquor by using UV spectrophotometry.[Method] With the ethyl acetate as the extractant,the absorption peak of avilamycin ethyl acetate liquor at the wavelength of 200～400 nm was scanned,the avilamycin ethyl acetate liquor with series concn.was precisely prepared,the standard curve was drawn and measured under the optimum wavelength.Then the fermentation samples were made for 3 centrifugation treatments to get the sample liquor.The sample liquid was made for the reproducibility and stability research and the calculation of recovery rate and the antibacterial effect of samples were verified by using the strain zone method.[Result] Selecting the wavelength of 275 nm as the quantitation peak,When the avilamycin ethyl acetate liquor concn.was in the range of 0.5-12.0 mg/L with pH value of 4.6,its linear relationship of absorbance and concn.was good.The linear regression equation was y=15.968 0x＋0.030 7 and the correlation coefficient was 0.995 5.The sample liquor kept stable under the room temperature for 24 h and the average recovery rate was 102.13%.[Conclusion] The test proved that it was feasible to measure the avilamycin content in the fermentation liquor by using UV spectrophotometry.