摘要
建立了4种水果中丁酰肼残留量的液相色谱-电喷雾串联四极杆质谱检测方法。样品经匀浆提取净化后,采用LC-MS/MS法,ESI(+)电离方式,多反应监测(MRM)定量,用Vensil HILIC色谱柱,以含0.1%甲酸的水和乙腈为流动相进行洗脱。结果表明:丁酰肼在0.01～1.0mg/L质量浓度范围内呈现良好的线性,相关系数为0.9991,方法的检出限为2.5μg/kg,当添加质量分数为0.01、0.1、0.5mg/kg时,4种水果中丁酰肼的平均回收率为77.7%～103.7%,相对标准偏差为2.0%～9.3%。
A method for determination of daminozide residue in four fruits by liquid chromatography-ESI tandem mass spectrometry （LC-MS/MS） has been developed. After sample was extracted and cleaned up, daminozide was detemined by LC/MS/MS-ESI（-）, quantified with multiple reaction monitoring（MRM） mode. The separation was performed on a Vensil HILIC column with a gradient system of water （containing 0.1% formic acid）-acetonitril as mobile phase. The results showed that the calibration linear range of daminozide was from 0.01 to 1.0 mg/L with a correlation coefficient of 0.9991. The detection limit was 2.5 gg/kg. Average recoveries （spiked at the levels of 0.01, 0.1, 0.5 mg/kg） range from 77.7 - 103.7%, the relative standard deviations was between 2.0 and 9.3 %.
出处
《农药》
CAS
北大核心
2010年第3期191-193,共3页
Agrochemicals
基金
北京市科委项目(Z07090500550702)
