WR-2721药对放烧复合伤预防效应的实验观察

PROTECTIVE EFFECT OF WR 2721 ON THE COMBINED RADIATION BURN INJURY IN MICE

研究ＷＲ２７２１（氨基硫醇类药）对放烧复合伤的预防效果及其对造血功能的保护作用。结果证明：ＷＲ２７２１能明显提高放烧小鼠的活存率，并延长死亡动物的活存时间。用ＷＲ２７２１预防后，放烧小鼠的ＬＤ５０由对照组的５．１９Ｇｙ增大到９．５３Ｇｙ，ＤＲＦ为１．８４。ＷＲ２７２１对放烧小鼠的预防作用以２００ｍｇ／ｋｇ照射前立即给药效果最好。放烧小鼠用ＷＲ２７２１预防后，与对照组相比，外周血白细胞数和骨髓有核细胞数下降幅度少，恢复快，骨髓粒单系祖细胞伤后第１ｄ和７ｄ及骨髓造血干细胞伤后第７ｄ和１４ｄ明显高于对照组。说明，ＷＲ２７２１对放烧小鼠的预防作用，至少部分是通过保护造血功能而实现的。

Protective effect of agent WR 2721 on hematopoiesis and prophylaxis for the combined radiation burn injury were investigated in the present study. It was found that pretreatment with WR 2721 could significantly raised the survival rate and suwival time of mice with the combined radiation burn injury. Prophylactic use of WR 2721 resulted in an increase of LD50 value from 5.19 Gy of the control to 9.53 Gy of the pretreatment. DRF (dose reduction factor) was 1.84. Prophylactic effect of WR 2721 was gained by the immediate use before irradiation with the dose of 200 mg/kg. Pretreatment of WR 2721 in mice with the combined injury brought about a lesser decrease in peripheral WBC count and an earlier recovery compared with the control. Bone marrow myelocyte and monocyte counts were markedly higher than the control at day 1 and 7 post injury and bone marrow hematopoietic stem cell count was significantly higher than the control at day 7 and 14. Prophylactic effect of WR 2721 on the combined radiation burn injury was at least partially due to its protective effect on hematopoiesis.