摘要
以深层发酵培养的虎奶菇(Pleurotus tuber-regium)菌丝体和发酵液为材料,提取胞内和胞外多糖,研究对藻类细胞的凝集作用.结果表明:胞内多糖使蛋白核小球藻(Chlorella pyrenoidosa)和捷克小球藻(Chlorella luteorividis)产生凝集的最小凝集质量浓度分别是23.3,186.4μg/mL,而胞外多糖使上述2种小球藻产生凝集的最小凝集质量浓度分别是3.3,211.3μg/mL,对海洋小球藻(Chlorella sp.)无凝集反应.
Extracellular and intracellular crude polysaccharides were extracted from Pleurotus tuber-regiurn under the culture of submerged fermentation and tested for agglutination with algal cells. The results showed that intracellular and extracellular crude polysaccharides could agglutinate Chlorella pyrenoidosa and Chlorella luteorividis, but they had no agglutination with Chlorella sp.. The minimal concentration of intracellular crude polysaccharides, which could agglutinate Chlorella pyrenoidosa and Chlorella luteorividis cell suspension, were 23.3,186.4 μg/mL , respectively. The minimal concentration of extracellular crude polysaccharides, which could agglutinate Chlorella pyrenoidosa and Chlorella luteorividis cell suspension, were 3.3, 211.3μg/mL, respectively.
出处
《福建师范大学学报(自然科学版)》
CAS
CSCD
北大核心
2010年第2期106-110,共5页
Journal of Fujian Normal University:Natural Science Edition
基金
福建省科技厅重点资助项目(2008N0034)
福建省自然科学基金资助项目(2007J0326)
福建省教育厅资助项目(JA07046)
关键词
虎奶菇
多糖
藻细胞
凝集作用
Pleurotus tuber-regium
polysaccharide
alagal cells
agglutination