摘要
目的通过观察当归拈痛汤对佐剂性关节炎大鼠肿瘤坏死因子(TNF-α)的影响,评价当归拈痛汤治疗类风湿关节炎(RA)的疗效。方法将60只雄性Wistar大鼠随机分为正常组、模型组、高剂量组、中剂量组和低剂量组,采用弗氏完全佐剂造模,观察不同时间点各组大鼠的疼痛指数、肿胀指数等;在灌服当归拈痛汤18 d后,用ELIASA法检测血清TNF-α的水平。结果致炎后不同时间点各组关节肿胀度、疼痛指数比较均有显著性差异;与模型组比较,各用药组的TNF-α水平显著降低(P<0.05和0.01)。结论当归拈痛汤可抑制RA大鼠过高的炎性细胞因子TNF-α分泌,能明显抑制大鼠佐剂性关节炎病变。
Objective It is to observe the effect of Danggui Niandong decoction on TNF- α in rats with adjuvant arthritis to evaluate this decoction＇s curative effect on rheumatoid arthritis. Methods Sixty male Wistar rats were randomly divided in- to normal group, model group, high-dose group, middle dose group and low dose group. The models were made by Freund＇ s complete adjuvant. Pain index, swelling index and so on were observed in each group at different time points. The serum level of TNF α was determined ELIASA after 18 days＇ treatment with Danggui Niantong decoction by intragastric adminis- tration. Results There were significant differences in swelling degree and pain index of joints in every group in different proin- flammatory times. Compared with the model group, the level of TNF- α in every treatment group significantly lowed down （P 〈 0.05 and P 〈 0.01 ）. Conclusion Danggui Niantong decoction can inhibit the secretion of inflammation factor TNF- α, it can obviously inhibit the pathologic changes of adjuvant arthritis in the rats.
出处
《现代中西医结合杂志》
CAS
2010年第10期1187-1188,1236,共3页
Modern Journal of Integrated Traditional Chinese and Western Medicine
基金
新疆维吾尔自治区科技厅少数民族培养基金(200723106)
