顶空固相微萃取气质联用测定蒜油挥发性成分

Analysis of volatiles from garlic oil by headspace-solid phase microextraction-gas chromatography-mass spectrometry

以蒜油挥发性成分的总峰面积及总峰面积与峰数的比值为评价指标,比较了不同萃取头、吸附温度、吸附时间及解吸时间对固相微萃取的萃取效率的影响,得到最佳萃取条件:85μmPA萃取头、吸附温度40℃、吸附时间35min、解吸时间3min,结果表明:固相微萃取技术有效地吸附了蒜油中的挥发性成分,经NIST质谱数据库检索和文献对照,共确定27种成分,并且不需溶剂,分析简单、快速、经济。

A method for the determination of volatiles from garlic oil using headspace solid phase microextraction （HS-SPME） and gas chromatography-mass spectrometry（GC/MS） was presented.By total peak area and ratio of total peak area to number of peaks used as the main evaluating indexes, the analysis parameters of different SPME fibers, extraction time, extraction temperature, desorption time were optimized.The optimum extraction conditions were obtained as follows：85μm PA fiber,exposure for 35min at 40℃,and desorption for 3min at 250℃. The results showed that SPME was effective to analysis of the volatiles in garlic oil,twenty-seven compounds were identified.The method is simple,quick,solventless and inexpensive.