摘要
用还原胺化法制备大分子葡聚糖-牛血清蛋白(BSA)拟糖蛋白抗原,通过SDS-聚丙烯酰胺凝胶电泳结合考马斯亮蓝和过碘酸-品红两种染色方法验证偶联物的生成。考察不同反应条件(葡聚糖T40的氧化度,偶联pH,氧化葡聚糖和蛋白质的比值,偶联时间)对偶联反应的影响。确定拟糖蛋白抗原的最佳偶联条件为:葡聚糖T40/氧化剂NaIO4=1/120(物质的量比),偶联溶液pH8.0,葡聚糖T40/BSA=1/1(物质的量比),偶联时间24h。并将此反应条件用于T10和T100拟糖蛋白抗原的合成。
A high active yield dextran-BSA conjugate antigen was prepared by Reductive Amination Method.The covalent attachment of dextran to protein was confirmed by SDS-PAGE stained Coomassie brilliant blue and PAS-fuchsin. Different reaction conditions including oxidation of dextran, pH, dextran-and BSA concentrations, reaction time various, showed different effects on the entire reaction.The optimum condition was dextran/NalO4 = 1： 120, pH 8.0, dextran/BSA = 1：1 and 24h.And this reaction conditions to be used for synthesized other neoglycoproteins antigens contained T10 and T100.
出处
《食品工业科技》
CAS
CSCD
北大核心
2010年第3期219-221,224,共4页
Science and Technology of Food Industry
基金
省部产学研结合项目
关键词
葡聚糖
拟糖蛋白
抗原
偶联条件
dextran
neoglycoprotein
antigen
reaction conditions
