摘要
采用紫外分光光度法测定甘蔗糖蜜中5-羟甲基糠醛(5-HMF)含量,以5-羟甲基糠醛为标准品,在284nm波长处对糖蜜样品进行了定量分析。测定结果表明,回归方程为Y=0.02046+0.1145X,R=0.9995,线性范围0～15μg/mL,测定精密度RSD为1.27%,重现性良好。平均回收率为97.91%,回收率RSD为1.67%(n=6),检出限为0.08μg/mL。
The content of 5-hydroxymethyl-2-furfural （5- HMF） in molasses was measured by ultraviolet spectrophotometry（UV） using 5-hydroxymethyl-2-furfural as a standard sample at the wavelength of 284rim.The experimental results demonstrated that the regression equations were ： Y = 0.02046 ＋ 0.1 145X, R = 0.9995, the linear range was 0-15μg/mL,and the precision of method was 1.27% ;the average recovery rate was 92.91 %, RSD was 1.67 % （ n = 6）, and the detection limit was 0.08μg/mL.
出处
《食品工业科技》
CAS
CSCD
北大核心
2010年第3期365-367,共3页
Science and Technology of Food Industry
关键词
糖蜜
5-羟甲基糠醛
紫外分光光度法
molasses
5-hydroxymethyl-2-furfural
ultraviolet spectrophotometry
