荔枝中水溶性维生素的毛细管电泳快速分离分析

Fast separation and analysis of water-soluble vitamins in lichee by capillary electrophoresis

本研究通过双模对接高压电源实现了毛细管电泳中0～40 kV及以上的超高电压。通过考察电压、缓冲溶液浓度、pH等对分离的影响,确定了优化的实验条件。结果表明,在40 kV的超高电压下,缓冲液为25 mmol/L硼砂-H3BO3溶液(pH 8.8),8种水溶性维生素(VB1、VB2、VB6、VC、D-泛酸钙、D-生物素和烟酸、叶酸)在2.2 min内获得了较好的基线分离。同时对荔枝中的水溶性维生素进行定量分析得到了令人满意的结果。

In capillary electrophoresis,0～40 kV（even higher） voltage can be reached by connecting double-model high voltage power supply.In the article,the separating conditions,such as voltage,the concentration of buffer,pH etc,were optimized during the experiments.The results showed that eight kinds of water-soluble vitamins（VB1,VB2,VB6,VC,Calcium D-Pantothenate,D-Biotin,Nicotinic acid and Folic acid） could be baseline separated in 2.2 min at 40 kV high voltage,in 25 mmol/L sodium tetraborate buffer solution（pH 8.8）.At the same time,water-soluble vitamins in lichee were quantified.