摘要
研究了红景天中总黄酮提取率的影响因素,先经单因素实验初步确定影响因素,再用Box-Behnken响应面法建立影响因素的二次回归模型,通过对二次回归模型求解编码转换得知,液料比40:1(ml/g),酶添加量1.2%,酶解时间4.5 h,酶解温度45℃,pH5,超声时间为12 min,超声功率为250w中的高档,超声次数为3次,在这最优条件下总黄酮提取率为3.99%。
The factors influencing extraction of flavones from Rhodiola sacra were studied by single - factor experiments. Then response surface methodology （ RSM ） of Box - Behnken was used to optimize the above critical factors. The optimum levels of the factors were calculated through regressive method and the result as follows： samples were hydrolyzed by using the ratio of liquid to material 40 ： 1 （ ml/g）, cellulase of 1.2% , ultrasonic time of 12min for 3 times, ultrasonic powerful strength of 250W, at45 ℃and pH 5.0 for 4.5hours. Under the optimum levels , the extraction rate of flavones was up to 3.99%.
出处
《氨基酸和生物资源》
CAS
2010年第1期59-63,共5页
Amino Acids & Biotic Resources
