烤烟品种K326突变株抗烟草普通花叶病(TMV)的研究初报

Preliminary study on TMV resistance in K326 mutant

通过2006～2008年的田间试验，进行了烤烟惦种K326突变株CQY材料对烟草普通花叶瘸（TMV）抗性研究。结果表明：接种TMV后第7dK326、CQY和Coker176心叶表现系统斑驳的发病率分别为99．10％、42．54％和1．18％；接种TMV后第14d和第21d，CQY和Coker176发病率与第7d相比略有增加，K326发病率为100％，方差分析表明3个处理间差异极显著（P〈0．01）。CQY没有表现系统斑驳的烟株在接种叶片部位表现过敏性坏死斑，症状与Coker176的过敏性坏死斑相似。CQY病情指数从第7d的10，64快速发展到笫14d的35．11，最后到第21d的39．63，方差分析结果显示，接种TMV后第7d、第14d和第21dK326、CQY和Coker1763个处理间的病情指数差异均为极显著（P〈O．01）。农艺性状测量结果表明，CQY材料与K326在株高、叶片数、茎嘲、节距等差异不显著，CQY材料较K326叶片变短变宽；产量产值与K326差异不显著（P〉0．05）。

TMV resistance of a K326 mutant was investigated by field experiment in 2006 - 2008. Results showed that TMV incidence after 7 days inoculation in K326, CQY, and Coker176 was 99.10%, 42.54%, and 1.18%, respectively. TMV incidence in CQY and Coker176 on dayl4 and 21 was slightly higher than that at day7, and that of K326 was 100%. Significant difference （P 〈 0.01） in incidence was found among K326, CQY, and Coker176. In inoculation position, CQY without mosaic symptom caused necrotic spots witch were similar to those of Coker176. Disease index of CQY increased from 10.64 to 35.11 at the initial stage （7-14）, and was 39.63 on day 21. Significant differences （P 〈 0.01） were found in disease index among K326, CQY, and Coker176, on day 7, 14, and 21. It indicated that agronomic traits including plant height, number of leaves, stem girth, and node distance had no significant differences between K326 and CQY. Leaf of CQY was significantly shorter and wider than that of K326. Results of multiple comparison showed no significant （Ip 〉 0.05） difference in yield and production value between K326 and CQY.