大鼠再生肝8种肝脏细胞的生物氧化相关基因转录谱预示的生理活动

Transcription Profiles of Biological Oxidation-Related Genes in Rat Eight Kinds of Regenerating Liver Cells

为了解大鼠肝再生中8种肝脏细胞的生物氧化相关基因转录谱及其预示的生理活动,用Percoll密度梯度离心结合免疫磁珠分选方法分离大鼠再生肝的肝细胞、胆管上皮细胞、卵圆细胞、星形细胞、窦内皮细胞、库普弗细胞、陷窝细胞和树突状细胞等8种细胞,用Rat Genome2302.0芯片等检测生物氧化相关基因在上述细胞中表达变化,用H-Cluster等软件及生物信息学和系统学生物等方法分析它们的表达模式及预示的生理活动.结果表明:38个生物氧化相关基因在大鼠肝再生中发生了有意义表达变化,相应细胞的基因数为6,32,0,2,3,1,3,2个.肝再生启动阶段和进展阶段的NAD+合成增强,从NADH到O2的电子传递及ATP合成增强.终止阶段的FADH2分解减弱,从FADH2到O2的电子传递减弱.结论:大鼠肝再生与生物氧化密切相关.

To study the transcript atlas of biological oxidation-related genes in rat 8 liver cell types including hepatocytes,bile duct epithelial cells,oval cells,hepatic stellate cells,sinus endothelial cells,Kupffer cells,pit cells and dendritic cells from the regenerating liver,they are isolated respectively by Percoll density gradient centrifugation combined with immunomagnetic beads method. Their expression changes in the regenerating livers are detected by Rat Genome 230 2.0 array,and the expression patterns and the predicted physiological activities of biological oxidation-related genes are analyzed by Cluster program,and the methods of Bioinformation and Systematic biology.The results show the meaningful expression changes of totally 38 genes associated with biological oxidation during liver regeneration,and the gene number of the corresponding cells is 6,32,0,2,3,1,3 and 2,respectively. It is concluded that the production of NAD＋ and ATP,the electronic delivery of respiratory chain from NADH to O2 are enhanced at the priming and progressing phases of liver regeneration; FMNH2 decomposition and the electronic delivery of respiratory chain from FADH2 to O2 become weak at terminal phase. Conclusion：the rat liver regeneration is closely related to the biological oxidation.