苦参中总生物碱的富集工艺研究

Study on Accumulate Total Alkaloid From Sophora Flavescens Ait

目的:研究用阳离子交换树脂富集苦参中总生物碱的工艺条件。方法:采用732阳离子交换树脂对苦参中总生物碱进行富集,以苦参中总生物碱含量为考察指标,采用分光光度法测定总生物碱含量。结果:苦参中总生物碱的最佳富集工艺条件为:上样溶液质量浓度为0.4g·mL-1,以1%Na2CO31BV.h-1洗脱,收集17BV。结论:732阳离子交换树脂对苦参中总生物碱的吸附能力较好,适用于苦参中总生物碱的富集。

Objective：To study the technology for accumulate total alkaloid from Sophora flavescens Ait by Means of Cation Exchange Resin.Methods：accumulate total alkaloid from Sophora flavescens Ait was performed by Means of 732 Cation Exchange Resin,and the content of total alkaloid from Sophora flavescens Ait was determined as an index by spectrophotometry to choose the optimal technological parameters.Results：To accumulate total alkaloid with 17 BV 1%Na2CO3 and the destiny of solution was 0.4 g·mL-1.Conclusion：732 Cation Exchange Resin has the good ability of absorbing the total alkaloid from Sophora flavescens Ait and can be used to accumulate total alkaloid from Sophora flavescens Ait.