黄花蒿中青蒿素含量的紫外分光光度法测定

Determination of Artemisinin in Artemisia Annua with UV Spectrophotometry

建立了黄花蒿中青蒿素含量的紫外分光光度法测定。并对该方法提取溶剂的选择、黄花蒿提取物与NaOH反应情况及最佳青蒿素碱转化条件进行了讨论。以正己烷或石油醚等非极性类溶剂作提取剂,对青蒿素的提取率较高,提取物中杂质含量较低;提取物中非青蒿素成分与NaOH反应能快速完成,且一般不引起292nm附近吸收值的变化,因此不干扰青蒿素含量的测定;青蒿素碱转化反应可选择在25℃下与0.3%的NaOH作用30min,或50℃下与0.2%的NaOH作用20min。方法的相对标准偏差RSD=1.84%,相关系数r=0.9995,回收率为98%—102%。

The method for determination of artemisinin in artemisia annua with UV spectrophotometry was established. And the selection of solvent used in extraction, the reaction of artemisia annua extracts with NaOH and the optimal condition for base conversion of artemisinin were discussed. With nonpolar solvents such as hexane or mineral ether as extracting reagent,the extracting rate of artemisinin is higher, the content of impurity is lower, among extracts non-artemisinin components can react quickly with NaOH and not cause variations in absorption value around 292nm, so they will not disturb determination of artemisinin. Base conversion xeaction of artemisinin can be conducted under following conditions： reacting with 0. 3% NaOH at 25℃ for 30 minutes or with 0.2% NaOH at 50℃ for 20 minutes,the relative standard deviation （RSD） is 1.84%,correlation coefficient r=0. 9995,and the recovery is 98%--102%.