光镊捕获金福菇孢子的拉曼光谱分析

Analysis of the Raman Spectrum of Single Tricholoma Lobayense Spores Trapped by Laser Tweezers

使用光镊拉曼光谱系统俘获悬浮在生理盐水中的单个金福菇孢子,激发并收集其拉曼信号,结果显示单个金福菇孢子的拉曼光谱基本能呈现其内含物的组成和结构信息,脂类物质是其主要成分。同种栽培方式的3个不同菌株单个金福菇孢子的平均拉曼光谱信号基本一致,分别对其进行多元统计分析(PCA),无法区分其拉曼光谱信号;同一菌株不同栽培方式的单个孢子平均拉曼光谱的差异光谱表明其主要成分相同,都以脂类物质为主;而源自于脂类1743、1655、1442、1125、1080、1070、876、728cm-1等峰的信号强度基本一致,说明其脂类的含量基本相同。从单细胞拉曼光谱角度初步分析金福菇孢子,为光镊拉曼光谱技术研究食用菌孢子萌发机理提供有意义的参考。

Laser tweezers and Raman spectroscopy system were applied to measure the Raman spectrum of single tricholoma lobayense spores which immersed in salt solution. The mean spectra from single spores of three stains of tricholoma lobayense showed large similarity. Lipoids were the main component according to the spectrum of the single spore. Principal components analysis could not distinguish the spores from different strains. The spectra of single spores collected from different cultivation mode are also similar. Laser tweezers Raman spectroscopy system would be a useful tool in study of the mechanism of spore germination.