氢化物发生-原子荧光光谱法测定7种中草药中不同形态的水溶性砷

Determination of Arsenic Species in Seven Chinese Herbs by Microwave Extraction-Hydride Generation Atomic Fluorescence Spectrometry

微波提取样品后,使用树脂分离不同形态的砷,以盐酸为测定介质,硫脲-抗坏血酸为预还原剂,硼氢化钾为还原剂,用氢化物发生-原子荧光法测定7种驱虫类中草药中的不同形态砷含量。砷含量在10—100ng/mL浓度范围内线性关系好,回收率在91.4%—106.4%之间。本方法简便、快速、灵敏、准确,可用于中草药中砷的形态测定。

The content of arsenic species in seven Chinese herbs were determined by hydride generation-atomic fluorescence spectrometry with ion exchange resin separation. The samples were treated by microwave extraction. Hydrochloric acid was the medium, thiourea-ascorbic acid was the pro-reducing agent,potassium borohydride was the reducing agent. In the range of 10--100ng/mL, arsenic had a good linearity. The recovery was from 91.4 % to 106.4 %. This method is simple, rapid, sensitive and accurate,and it can be used to determine arsenic species in Chinese herbs.