裸玻碳电极直接电化学发光测定诺氟沙星含量

Direct Electrochemiluminescenct Method for the Determination of Norfloxacin Based on a Bare Glassy Carbon Electrode

基于诺氟沙星能够显著增强过硫酸根的电化学发光信号,建立了一种灵敏、简便而且价廉的测定诺氟沙星含量的新方法。在最佳实验条件下,诺氟沙星浓度在0.141—6.33μg/mL范围内,与电化学发光信号强度呈良好的线性关系,线性回归方程为IECL=116.4+73.5CNFLX(μg/mL),r=0.9968,检出限为0.039μg/mL(S/N=3)。对4.22μg/mL的诺氟沙星平行测定11次,RSD为1.3%,表明该方法具有良好的重现性,并成功地应用于人尿样中诺氟沙星含量的测定。

A novel electrochemiluminescent （ECL） method for the determination of norfloxacin （NFLX） was developed based on the ECL enhancement of peroxydisulfate（S2O8^2-） by NFLX. Under the optimal conditions, the ECL intensity increased with the concentration of NF＇LX. A linear regression equation （IECL= 116.4＋ 73. 5CNFLX= 0. 9968） was obtained in the range of 0. 141- 6.33ug/mL. The limit of detection （S/N= 3） was 0. 039ug/mL with the relative standard deviation （n= 11） of 1.3% for 4.22ug/mL NFLX. The proposed method is sensitive,simple and cheap,and is applied to detect NFLX in human urine successfully.