一株抗氧化活性酵母菌产多糖的纯化与结构分析

Analysis of Structure of Purified Polysaccharide from Yeast Strain with Antioxidant Activity

研究了Pichia fermentans酵母菌产多糖YP5的单糖组成和结构。将酵母菌进行超声破碎后,采用乙醇沉淀法得到粗多糖,然后进行DEAE-纤维素色谱柱分离,得到均一的水溶性多糖YP5。经气相色谱分析表明,糖基由鼠李糖、阿拉伯糖、木糖、甘露糖、葡萄糖与半乳糖组成,其摩尔比为9.51∶1.33∶2.31∶0.94∶84.93∶0.96。乌氏粘度计法测得多糖的分子量为8.3×104。结合红外光谱和核磁共振1HNMR分析表明,该多糖YP5具有多糖特征性的红外吸收峰,存在酰胺结构,主要由β-D-葡聚糖组成。

The monosaccharide composition and the structure of polysaccharide from Pichia fermentans were analyzed. The yeast cells were broken with ultrasonic disruption and the crude polysaccharide was precipitated by addition of ethanol. Then,the crude polysaccharide was separated by diethylaminoethyl（ DEAE） cellulose column separation and an acquisition of uniform water-soluble polysaccharide yeast polysaccharides 5（ YP5） was obtained. The results of gas chromatographic analysis indicated that the glycosyl was made of rhamnose, arabinose,xylose,mannose,glucose and galactose,whose molar ratio was 9. 51∶1. 33∶2. 31∶0. 94∶84. 93∶ 0. 96,respectively. The molecular weight of polysaccharides YP5 was 8. 3 × 104 measured by Ubbelohde viscosity method. Combination of IR and 1H NMR analysis showed that the YP5 owned characteristic infrared absorption peaks of polysaccharide and contained amino groups. The β-D-glucan was main glucosidic linkage of polysaccharides PY5.