何首乌主要成分大黄素、大黄酸和二苯乙烯苷对肝细胞、肝癌细胞的影响

Influence of main component of Heshouwu such as emodin,rhein and toluylene glycoside on hepatic cells and hepatoma carcinoma cell

目的通过观察何首乌主要成分大黄素、大黄酸和二苯乙烯苷对肝细胞、肝癌细胞的影响来探讨引发何首乌不良反应的物质基础，为规范中药合理应用提供理论依据。方法用MTT法观察何首鸟主要有效成分大黄素、大黄酸和二苯乙烯苷在不同浓度下作用不同时间对肝细胞和肝癌细胞的影响。结果蒽醌类化合物大黄素、大黄酸在终浓度6．25～50μmol／L，随着浓度的增加、作用时间的延长对L-02细胞和BEL细胞的损伤加大、抑制率增加；二苯乙烯苷在终浓度5～400μmol／L，随着浓度的增加、作用时间的延长对L-02细胞和BEL细胞的影响不明显，无显著性差异。结论大黄素、大黄酸是何首鸟引发不良反应一肝毒性的主要成分，在高浓度、长时间作用下有细胞毒作用，二苯乙烯苷在5～400μmol／L作用不同时间对肝细胞和肝癌细胞的影响不明显，无细胞毒作用。

Objective It is to approach the material base of untoward reaction of Heshouwu through observing the influence of main component of Heshouwu such as emodin, rhein and toluylene glycoside on hepatic cells and hepatoma carcinoma ceil, and to provide the theoretic evidence for rational use of traditional Chinese medicine. Methods The influences of emodin, rhein and toluylene glycoside in different concentration and different time on hepatic cells and hepatoma carcinoma cell were observed with MTT methods. Results The more the concentration of anthraquinones-emodin, rhein increasd during 6.25- 50μmol/L, and the longer time action, the more damage on L- 02 and BEL cells. But the influence of toluylene glycoside on L - 02 and BEL cells were not significant with the concentration increased. Conclusion Emodin and rhein are the primary components of Heshouwu occurred liver-toxicity, there are cytotoxin under high concentration and long period action on L- 02 and BEL cells and that toluylene glycoside have no significant influence during 5- 400μmol/-L and have no cytotoxin charictristics.