I型鸭肝炎病毒RT-PCR检测方法的建立

Establishment of RT-PCR method for detecting Duck Hepatitis Virus I

根据GenBank发表的DHVI的基因序列,设计了1对针对DHVI保守区的特异性引物。通过对反应条件的优化,建立了鸭病毒性肝炎病毒快速鉴别诊断的RT-PCR方法,扩增片段大小为632bp。试验表明,RT-PCR法具有很强的特异性,且该方法的敏感性明显高于常规的检测方法。对鸭病毒性肝炎的病料检测证明,该方法能有效鉴别DHV,特异性良好。

According to the conserved gene sequence of Duck Hepatitis Virus I（DHV I） in GenBank,one pair of the specific primers were designed. A rapid diagnostic technique of RT-PCR was established by optimizing the reaction condition. The DNA segments amplified were 632 bp. The experiments had proved that RT-PCR possessed a high specificity. And the sensitivity test results indicated that the RT-PCR was more sensitive than the conventional method. Detection of field samples from viral diseased ducks also confirmed that the method of RT-PCR could effectively identify the infection of DHV.