牛膝多肽对NMDA诱导的视网膜神经节细胞损伤的保护作用

The protective effects of Achyranthes bidentata polypeptides on retinal ganglion cells induced by exposure to NMDA

目的:观察牛膝多肽(ABPP)对N-甲基-D-天冬氨酸(NMDA)诱导的转基因视网膜神经节细胞RGC-5细胞株损伤的保护作用。方法:在体外培养的RGC-5中加入不同浓度的牛膝多肽(0.05、0.5、5.0μg/ml)预保护12小时,用NMDA(100μmol/L)诱导细胞损伤,同时设正常对照组、单纯损伤组和地卓西平(MK-801)对照组,倒置显微镜下观察细胞形态;MTT比色法测定细胞存活率;Hochest染色检测细胞凋亡。结果:NMDA可诱导RGC-5损伤,与单纯损伤组比较,0.5和5.0μg/ml ABPP可提高RGC-5存活率,减少细胞凋亡(P<0.05)。结论:ABPP可抑制NMDA诱导的视网膜神经节细胞凋亡,保护视网膜神经元。

Objective：To investigate the protective effects of Achyranthes bidentata polypeptides on retinal ganglion cells induced by exposure to NMDA.Methods：In vitro cultured RGC-5 were pretreated with ABPP（0.05,0.5,5.0μg/ml）for 12h and exposed to NMDA（100μmol/L）for 30min.The normal RGC-5,NMDA treated and MK-801 treated RGC-5 were used as control.The protective effects were observed by invert microscope,MTT assay and Hoechst staining.Results：Exposure of RGC-5 to NMDA may induce neuronal injury.Pretreated with ABPP（0.5 and 5.0μg/ml） greatly improved the viability of RGC-5 and attenuated the apoptosis of RGC5.Conclusion：ABPP could inhibit the apoptosis of retinal ganglion cells induced by exposure to NMDA and provide neuroprotective effects on retinal ganglion cells.