小鼠后肢缺血再灌注后背根神经节超微结构的改变

The ultrastructure changes of dorsal root ganglion after hindlimb ischemia-reperfusion in mice

目的:研究小鼠后肢缺血再灌注对背根神经节(DRG)形态结构的影响。方法:采用暂时阻断小鼠髂总动脉起始处血流的实验模型,随机分为假手术组(A组)、缺血6h再灌注组(B组)和缺血8h再灌注组(C组),观察不同缺血时间再灌注后DRG的超微结构改变。结果:电镜下可见A组为正常细胞结构;B组细胞有局灶性核溶解发生,粗面内质网池扩张但其扩张程度远远低于C组。卫星细胞核孔消失,微血管内皮细胞暗细胞变。C组细胞核仁呈轻度溶解,线粒体变暗,卫星细胞明显增生,可见凋亡小体被卫星细胞吞入,微血管内皮呈暗细胞变,腔内可见崩解小球的空泡。腔隙间隔极度增宽,其间可见坏死细胞。结论:小鼠后肢缺血再灌注可引起DRG超微结构的损伤。

Objective： To explore the effects of dorsal root ganglion （DRG） after hindlimb ischemiareperfusion in mice. Methods： By using the model of occlusion and reperfusion of common iliac arteries of the mice, Male Kunming mice （20-25 g） were randomly divided into 3 groups with different treatments： Sham-treated control group （Group A）, ischemia for 6 h reperfusion groups （Groups B） and ischemia for 8 h reperfusion groups （Groups C）.The uhrastructure changes of DRG were observed. Results： Under electron microscopes those were observed, Group A： normal cyto-architecture; Groups B： cells had focal karyolysis, the rough endoplasmic reticulum cisterna was less dilated than groups C, nucleopore of satellite cell disap- pea.red, the vas eapiUare endothelial cells became dark cells; Groups C： entoblast light dissolved and chon- driosome became dark, satellite cell had hyperplasy and phagocytosed apoptotic body, vacuole of disaggre- gation bleb were found, laeouna spacing widen and cell necrosis were found. Conclusion： The mice＇s hindlimb isehemia-reperfusion can cause the ultrastructure damage of the DRG.