高产1,3-丙二醇菌株的诱变与筛选

Mutation and Screening of Klebsiella Pneumoniae for High Production of 1,3-Propanediol

为提高克雷伯氏肺炎杆菌产1,3-丙二醇(1,3-PD)的能力,利用紫外线对其进行诱变,建立了一种高效高通量的摇瓶传代连续富集培养筛选抗产物抑制菌株的方法,筛选出了4株抗1,3-PD抑制且高产1,3-PD的菌株,分别命名为KpⅠ、KpⅡ、KpⅢ和KpⅣ,其1,3-PD产量分别为74.80 g.L-1、77.98 g.L-1、76.04 g.L-1、73.50 g.L-1,比出发菌株提高了14.5%、19.4%、16.4%、12.5%。1,3-PD得率分别比出发菌株提高了8.1%、14.8%、11.5%、6.6%。

Based on ultraviolet light mutation method, the high efficient and throughput flask continuous enrichment culture screening technique was established, in order to increase the conversion ability of glycerol into 1,3-propanediol （1,3-PD） by Klebsiella pneumoniae. Four positive high production mutant strains, which were named KpⅠ , KpⅡ , KpⅢ, Kplg, were selected, and they not only could endure high-concentration 1,3- propanediol,but also produced high-concentration 1,3-propanediol. Compared to the parent strain,the 1,3-propanediol production capability of these four mutant strains was increased by 14.5%, 19.4%, 16.4%, 12.5%, increased to 74. 80 g ·L^-1 , 77.98 g ·L^-1 , 76.04 g ·L^-1, 73.50 g ·L^-1 ,respectively. And the 1,3-PD yield was increased by 8.1%, 14.8%, 11.5%, 6.6%.