胶体金免疫层析技术快速定量检测金黄色葡萄球菌肠毒素B

Rapid and Quantitative Detection of Staphylococcal Enterotoxin B with Colloid Gold Immunochromatography

目的建立胶体金免疫层析技术快速定量检测金黄色葡萄球菌肠毒素B的方法。方法利用胶体金标记和双抗体夹心免疫层析技术,建立金黄色葡萄球菌肠毒素B的快速检测方法,评价其特异性和敏感性,并拟合检测曲线进行定量检测;在牛奶样品中添加金黄色葡萄球菌肠毒素B作为模拟污染样品进行检测。结果该法可在5～10min内完成定性和半定量检测,检出限为8ng/ml,线性范围8～1000ng/ml。结论建立的检测金黄色葡萄球菌肠毒素B的胶体金免疫层析方法,能快速、灵敏、特异、准确地检测样品中的金黄色葡萄球菌肠毒素B,并可实现定量,适用于现场快速检测。

Objective To develop a rapid method for the detection and quantification of Staphylococcal enterotoxin B （SEB） in foods. Method Using colloid gold as a marker and utilizing double-antibody sandwich immunochromatographic assay to rapidly detect Staphylococcal enterotoxin B in foods, and to evaluate the specificity and sensitivity of the method. The quantification was realized by constructing a standard curve. The feasibility of the method was tested by adding SEB to various food samples, such as milk powder. Results The qualitative as well as semi-quantitative detection could be completed in 5-10 min. The limit of detection was 8 ng/ml; the linear range was from 8 ng/ml to 1000 ng/ml. The recovery rate was 120.82% and 120.23%. The specificity and sensitivity of the method was good, and the samples could be detected directly after simple processing. Conclusion The established colloidal gold immunochromatographic assay was able to detect SEB in food samples rapidly, specifically, sensitively and accurately, and was able to realize quantitative detection in the fields.