类风湿性关节炎患者杀伤细胞免疫球蛋白样受体基因多态性分析

Investigation of the killer cell immunoglobulin-like receptors gene polymorphisms of patients with rheumatoid arthritis

目的分析类风湿性关节炎(rheumatoid arthritis,RA)患者杀伤细胞免疫球蛋白样受体基因多态性,探讨其与类风湿性关节炎发病之间的关联。方法采用序列特异性引物聚合酶链反应(PCR-SSP)法,分析90例RA患者和200例健康对照组KIR基因。结果通过对RA病例组与正常对照组的9种KIR基因的分析,比较其基因频率发现:RA病例组KIR2DS4的基因频率显著低于对照组(P<0.05),差异具有统计学意义;其余各基因频率与对照组相比,P>0.05,差异无统计学意义。结论RA患者KIR2DS4基因可能与RA发病有关。

Objective To investigate the association between the killer cell immunoglobulin-like receptors gene polymorphisms and rheumatoid arthritis （R.A）. Methods DNA samples of 90 RA patients and 200 controls were genotyped by PCR-SSP. Results The gene frequency of K/R2DSK in RA was significantly lower than that in controls （/9〈0.05）. Conclusion KIR2DS4 gene may be associated with RA.