不同临床类型慢性乙肝患者乙肝病毒基因型检测

HBV genotypes in patients with HBV-related chronic liver diseases

目的探讨本院慢性乙型肝炎患者乙肝病毒基因型分布特征及与临床类型的关系。方法利用线性探针分析法进行乙肝病毒基因分型。结果52例慢性乙肝患者检出乙肝病毒基因型B型24例(46.2%),C型26例(50%),A、B混合型2例(3.8%),B、C两型均以男性多见,两型间性别比例差异无统计学意义(χ2=0.65,P>0.05);在慢性肝炎轻度、中度、重度、肝硬化及肝癌中,B和C基因型的分布差异无统计学意义(P>0.05);A、B混合型2例分别为慢性乙型肝炎轻度和慢性乙型肝炎中度。C型HBeAg阳性率高于B型,差异有统计学意义(P<0.05),HBVDNA载量两组间差异无统计学意义(P>0.05)。结论本组慢性乙肝患者的乙肝病毒基因型以B、C为主要亚型,且C型稍多,C型HBeAg阳性率高于B型。

Objective To study the distribution of hepatitis B virus （HBV） genotypes in patients with HBV-related chronic liver disease in our hospital and its relationship with clinical types. Methods The genotype of HBV was performed by the line probe assay （LiPA）. Results A total of 52 patients were included （39 males and 13 females） and their median age was 36 years （range 12-76 years）. Of the 52 patients, 24 （46.2%） were gen0.type B, 26 （50%） were genotypes C, and 2（3.8%） was mixed genotypes （A and B）. B and C genotypes were more common in males and there was no significant difference in gender proportion between these two genotypes （ x2=0.65, P〉0.05）. There was no significant difference for B and C genotype distribution among patients with HBV-related chronic mild, moderate and severe hepatitis, liver cirrhosis and hepatocellular carcinoma （P〉0.05）. Clinical types of two mixed genotypes （A and B） was chronic mild and moderate hepatitis, respectively. Serum HBeAg positive rate in patients infected with HBV type C was significantly higer than that in patients infected with HBV type B （P〈 0.05）. There was no significant difference for HBV DNA load between patients infected with HBV type B and C （P〉0.05）. Conclusion The HBV genotypes in patients with HBV-related chronic liver disease in our hospital are mainly type C, and then type B. Serum HBeAg positive rate in HBV type C -positive patients was significantly higer than that in HBV type B - positive patients.