重组别藻蓝蛋白对小鼠H_(22)肿瘤生长及COX-2、VEGF和PCNA表达的影响

THE EFFECT OF RECOMBINANT ALLOPHYCOCYANIN ON TUMOR GROWTH AND EXPRESSIONS OF COX-2,VEGF AND PCNA IN H_(22) MICE

目的观察重组别藻蓝蛋白（rAPC）对接种H22细胞小鼠的肿瘤生长及环氧化酶-2（COX-2）、血管内皮生长因子（VEGF）、增殖细胞核抗原（PCNA）表达的影响，并初步探讨其可能的机制。方法昆明小鼠随机分为5组（n=10），即模型组，rAPC低、中、高剂量组（25、50、100mg·kg-1·d-1），环磷酰胺组（CY对照组）。各组小鼠左前腋下皮下接种H22肝癌细胞，第2天除模型组外，其余4组分别以不同剂量的rAPC、CY灌胃，15d后处死，剥离出肿瘤，称质量，计算抑瘤率；用免疫组化法测定肿瘤组织中COX-2、VEGF和PCNA的表达。结果rAPC低、中、高剂量组小鼠Hzz肿瘤质量增长均较模型组缓慢，差异有显著性（F=8．926，q=3．794～7．684，P〈0．05、0．01），其抑瘤率分别为25．2％、36．7％、43．1％。rAPC各剂量组COX2、VEGF阳性表达均低于模型组（F=23．651、7．244，q=3．985～11．307，P〈0．01）；rAPC中、高剂量组PCNA阳性表达较模型组显著减少（F=164．380，q=1．513～31．265，P〈0．01）。结论rAPC可以抑制小鼠H22肿瘤的生长及COX2、VEGF和PCNA的表达。

Objective To observe the effect of recombinant allophycocyanin （rAPC） on tumor growth, COX-2, VEGF and PCNA in FI22 mice and approach its possible mechanism. Methods Kunming mice were randomized to five groups as the model group, low-, middle , and high dose rAPC groups （25,50,100 mg·kg^-1·d^-1, respectively）, and Cyclophosphamide group （control group） with 10 mice in each group. H22 cells were inoculated subcutaneously at left anterior armpit of the mice. A- nimals of the four groups, except the model group, were given different dosages of rAPC and CY on the second day and killed after 15 days. The tumors were removed, weighed, and tumor inhibition rate calculated; the expressions of COX-g, VEGF and PCNA in the tumor were determined immunohistochemically. Results The increase of tumor weight in low , middle-, and high-dose groups was slower than that of the control （F= 8. 926, q： 3. 794, P〈0.05）, the inhibition rate was 25.2 %, 36.7%, and 43.1%, respectively. The expressions of COX-2 and VEGF in the three different dose groups were lower than that of the model group （F= 23. 651 ;q=4. 476- 11. 307; P〈0.01）, the expression of PCNA in middle and high dose rAPC groups was less than the model （F=164.380;q：1.513 31. 265;P〈0. 01）. Conclusion rAPCinhibits tumor growth and expressions of COX-2, VEGF and PCNA in H22 mice.