黄河三角洲贝壳堤岛二色补血草生长和保护酶特性对盐胁迫的响应

Repones on Salt Stress on Characteristics of Growth and Protective Enzymes of Limonium Bicolor in Shell Islands of the Yellow River Delta

用不同浓度的NaCl溶液(0,50,100,200,300mmol/L)模拟盐胁迫,研究盐胁迫对二色补血草幼苗生长特性及保护酶系统的影响。结果表明:(1)100和200mmol/L盐胁迫下,根长和叶片数分别为对照的1.3,1.67倍,根(叶)鲜重、干重显著高于对照;根鲜重和叶干重在100mmol/L盐胁迫下最高,分别为对照的1.35,1.6倍;300mmol/L盐胁迫下,根长和叶片数均为对照的0.78倍,根(叶)鲜重、干重分别为对照的0.67(0.79)和0.85(0.69)。地上、地下生物量受盐胁迫的抑制程度大小不明显。(2)随着盐胁迫的增加,二色补血草叶片中SOD,POD,CAT3种保护酶活性均呈上升趋势,在盐浓度为300mmol/L时活性最大,分别为对照的2.12,1.83,2.57倍;MDA含量在盐浓度300mmol/L下,才显著高于对照,为对照的1.6倍。(3)从研究结果初步判定,二色补血草具有较强的耐盐性,可以在盐浓度300mmol/L以下的环境中生长。

The effects of salt stress on the characteristics of growth and protective enzymes were studied using Limoniumbicolor （Bunge） Kuntze. seedlings. Five treatments were arranged. 0, 50, 100, 200, and 300 mmol/L. Results showed that （1） At 100 and 200 mmol/L salt stress, the roots length and leaves number of Limonium bicolor were 1.3 and 1.67 times of those of the control, respectively. Fresh weight and dry weight of roots （leaves） were significantly higher than those of the control. Fresh weight of roots and dry weight of leaves were the highest at 100 mmol/L salt stress, which were 1.35 and 1.6 times of those of the control, respectively. At 300 mmol/L salt stress, roots length and leaves number were 0.78 times those of the control and fresh weight and dry weight of roots （leaves）, 0.67（0.79） and 0.85（0.69） times of those of the control. The effects of salt stress on aboveground biomass were not significant. （2） With increased salt stress, the three protective enzyme activities of superoxide dismutase （SOD）, peroxidase （POD）, and catalase （CAT） of Limonium bicolor increased, which were the highest at 300 mmol/L salt stress and were 2.12,1.83 and 2.57 times those of the control. The content of malondialdehyde （MDA） was 1.6 times of that of the control at 300 mmol/L salt stress. （3） The preliminary result showed that Limonium bicolor was a strong salt-tolerance plant and could grow at the salt concentration of 300 mmol/L.