水稻种子萌发过程中胚蛋白质差异表达分析

Differential Proteomic and Mass Spectrometric Analysis of Embryo Buds During Seed Germination in Rice (Oryza sativa L.)

通过采用IEF．SDS—PAGE双向电泳技术，对金稻1号水稻种子未萌发和萌发1-4天胚进行蛋白质分离。结果发现，金稻1号水稻种子未萌发和萌发1-4天胚在PDQuest图像分析软件可识别的蛋白质点约215、201、187、162、132个，其中表达量变化2．5倍以上的蛋白质点有22个。选取表达量变化2．5倍以上的12个差异蛋白质点进行胶内酶解，并进行肽质量指纹图谱及其生物信息分析，初步鉴定出6个蛋白质，分别为硫氧还蛋白；热休克蛋白；ATP合酶；LEA蛋白12；核苷二磷酸激酶；乙二醛酶。对这些蛋白质在水稻种子萌发过程中的作用进行了讨论。

IEF- SDS- PAGE two- dimensional gel electrophoresis （2-DE） technology was used to separated proteins from ungerminated seed and embryo buds of germinated seed at specific stages of 1, 2, 3, 4 days in rice （Oryza sativa L.） Jindao 1. The results showed that on the 2-DE gels stained by coomassie brilliant blue, PDQuest image software detected about 215, 201, 187, 162, 132 protein spots, of which 22 spots show more than 2.5-fold changes in abundance among ungerminated seed and embryo buds of germinated seed at specific stages of 1, 2, 3, 4 days in rice（Oryza sativa L.） Jindao 1. These 12 of 22 protein spots treated by tryptic in-gel digestion were characterized by matrix-assisted laser - desorption/ionization time of flight mass spectrometry （MALD I-TOF -MS） and bioinformaties analysis, peptidemass fingerprintings of all were obtained, of which 6 differential proteins were elementarily identified as thioredoxin peroxidase, heat shock protein, ATP synthase, LEA protein 12, nucleoside diphosphate kinase I （NDK I） （NDP kinase I）（NDPK I）and glyoxalase I respeetively. The potential functions of these proteins during seed germination were discussed.