番茄子叶和下胚轴离体再生体系建立

Establishment of Regeneration System of Cotyledon and Hypocotyl in vitro of Tomato

以番茄无菌苗子叶和下胚轴为外植体,研究其在MS附加不同浓度6-BA和NAA的培养基上的分化情况。结果表明:在不同培养基上,番茄子叶和下胚轴均能诱导出淡绿色的愈伤组织和分化形成不定芽,但在不同的培养基上,番茄子叶和下胚轴的出愈率和不定芽诱导存在明显差别。诱导番茄愈伤组织形成和芽分化的最佳培养基分别为:MS+6-BA2mg/L+IAA0.2mg/L和MS+6-BA2mg/L+IAA0.02mg/L,番茄不定芽生根培养基为:MS+IAA0.2mg/L。

The cotyledon and hypocotyl from tomato asepsis seedlings were cultured on the medium with different 6-BA and NAA concentrations to study their effects on the callus induction and shoot differentiation. The result indicated that some light green callus could be induced and adventitious buds could be differentiated in all kinds of medium, but there were obvious different in different medium. The optimum medium for tomato callus formation and bud differentiation respectively were MS＋6-BA 2 mg/L＋IAA 0.2 mg/L and MS＋6-BA 2 mg/L＋I AA 0.02mg/L. The best medium for inducing tomato root was MS ＋ IAA 0.2 mg/L.