薄层色谱和液相色谱法鉴别中兽药散剂中掺加的磺胺喹噁啉钠

Identification of Sulfaqiunoxline Sodium Adding to Herb Veterinary Drug Compound Preparation by TLC and HPLC

对磺胺喹噁啉钠采用硅胶GF254板为固定相,以正丁醇-浓氨水(15∶3)为展开剂,用薄层色谱鉴别中兽药散剂中磺胺喹噁啉钠分离较好,斑点显色清晰且无干扰,可用于中药散剂中非法添加物磺胺喹噁啉钠的快速筛选。采用C18柱为固定相,以甲醇-乙腈-水-冰乙酸(2∶2∶9∶0.2)为流动相,紫外检测波长为270 nm。液相色谱鉴别中药散剂中非法添加磺胺喹噁啉钠分离良好,无干扰峰,可用于中兽药散剂中非法添加物磺胺喹噁啉钠的鉴别。

TLC and HPLC method were established for the determination of sulfaqiunoxline sodium in herb veterinary drug compound preparation.TLC condition： Drug was separated on silica gel thin layer chromatography plate;developing agent was n-butanol/stronger ammonia water（15∶3）.HPLC conditons： the chromatography column was C18 column of 150 mm×4.6 mm,5 μm;mobile phase was methanol-acetonitrile-water-acetic acid（2∶2∶9∶0.2,V/V）;Determination was by UV detection at 270 nm.This meathod identifying sulfaqiunoxline sodium adding to herb veterinary drug compound preparation is successfully feasible.