Mta-1与VEGF在原发性肝癌组织中的表达及其相关性

Expression of Mta-1 and VEGF in Hepatocellular Carcinoma and Their Correlation

目的探讨肿瘤转移相关基因Mta-1、血管内皮生长因子(VEGF)在原发性肝癌组织和正常肝组织中的表达及其之间的相关性。方法免疫组化方法检测63例原发性肝癌组织和10例正常肝组织中转移相关基因(Mta-1)及血管内皮生长因子(VEGF)的表达;分析Mta-1和VEGF与原发性肝癌组织的肿瘤体积、病理类型、临床分期、淋巴结转移等临床病理参数之间的关系及Mta-1表达与VEGF的相关性。结果Mta-1与VEGF在肝癌组织中的表达阳性率均明显高于正常肝组织(χ2=10.59、6.42,P<0.01)。Mta-1在肝癌组织中的表达与肿瘤大小、临床分期、病理分级、有无淋巴结转移、有无门静脉癌栓、有无血道转移组织相关(P<0.01),VEGF在肝癌组织中的表达与临床分期、病理分级、有无淋巴结转移、有无门静脉癌栓、有无血道转移组织相关(P<0.01);在肝癌组织中Mta-1与VEGF蛋白表达呈正相关(rs=0.712,P<0.05)。结论肝癌组织中Mta-1和VEGF均呈过量表达,且二者表达存在协同促进关系,为肝癌的分子靶向治疗提供了新的靶点。

Objective To explore the expression of Mta-1 and VEGF in hepatocellular carcinoma tissues and normal hepatic tissues and their correlation. Methods The expression of Mta-1 and VEGF in 63 cases of hepatocellular carcinoma and 10 cases of normal liver tissues was examined by immunohistochemical method. The correlation of Mta-1 and VEGF expression and their correlation with the clinical parameters such as tumor size,pathologic type,clinical stage,and lymphnode matastasis of hepatocellular carcinoma were analyzed. Results The positive rates of Mta-1 and VEGF expression in hepatocellular carcinoma tissues were higher than those in normal liver tissues （χ2=10.59,χ2=6.42,P0.01）. The expression of Mta-1 in hepatocellular carcinoma tissues was related to tumor size,clinical stage,pathologic type,lymph node metastasis,portal venous invasion and distant metastasis （P0.01）. The expression of VEGF in hepatocellular carcinoma tissues was related to clinical stage,pathologic type,lymph node metastasis,portal venous invasion and distant metastasis （P0.01）. Positive correlation was found between VEGF and Mta-1 expression （rs=0.712,P0.05）. Conclusions There are over-expression of Mta-1 and VEGF in hepatocellular carcinoma tissues. Mta-1 expression combined with VEGF expression may have synergistic effect on hepatocellular carcinoma progression and provide a theoretical basis for application of molecular targeted drugs.