黄瓜cpSSR引物的多态性与通用性

Polymorphism and Transferability of Cucumber cpSSR Primers

从黄瓜叶绿体基因纽序列中设计15对以A或T为重复单元的微卫星引物，其中12对处于基因组非编码区，3对位于基因编码区。这15对引物在21份黄瓜材料中均有扩增产物，但只有非编码区的微卫星表现出位点多态性。每对引物可检测1～3个等位基因，平均2．0个，引物的多样性值（polymorphic information content PIC）从0至0．484，平均值0．202。15对引物在甜瓜和南瓜中均能通用，13对可在西瓜中通用。这些叶绿体引物可用于黄瓜及其近缘种的叶绿体遗传研究。

Fifteen microsatellite primers containing A/T repeat motifs were developed from the chloroplast genome sequence of cucumber （ Cucumis sativus L. ）. Of the primers, 12 located in non - coding regions and the rest three in coding regions. All the 15 primers had amplified products in 21 cucumber accessions, but only non - coding region located microsatellites displayed polymorphism. Each pair of primers could detect 1 -3 alleles （mean = 2.0） and their gene diversity value （polymorphic information content, PIC） was between 0 and 0.484 with a mean of 0. 202. All the primers could be transferred to melon and pumpkin, and 13 pairs could be transferred to watermelon. This study indicated these primers reported here could be used in the study of chloroplast heritance in cucumber and its related species.